![]() ![]() It saved my sanity from all of the questioning and uncertainty in the weeks leading up to baby. When my water broke at 39+1, and I took the litmus test again, there was zero doubt. It was incredibly reassuring every time I came back with a result around 6. A few weeks before that I had heard about using litmus tests, bought a pack and used them on a few occasions where I wasn't sure if a cough or sneeze had produced a little urine leakage or if it was my water. A $3 pack of litmus tests can save your sanity toward the end of pregnancy if you question whether every little leak was mild incontinence or your water breaking.įor some personal context: Toward the end of my last pregnancy I became one of the 10% of women whose water breaks prior to the onset of labor. The pH of urine is about 6 and the pH of amniotic fluid is about 7. Sharing just in case anyone else can benefit from this tip as well. Thank you to everyone who provided additional information. As others have pointed out the pH of urine is actually a wider range than I had posted, the test itself might lead to a false positive or negative and isn't considered reliable. This was something that worked for me but it isn't absolute and if you have any concerns that you are leaking aminiotic fluid, you should always consult your provider. This was a well-intentioned post but the test itself certainly has it's faults so do not read as medical advice. ![]() *If the Nitrazine Test is positive, but the Fern Test is negative, a second specimen should be collected and tested.Editing to add: a number of other redditors have been quick to point out the limitations of this suggestion. *If the Nitrazine Test is negative, but the Fern Test is positive, there is probable rupture of the membranes due to the Fern Test’s greater specificity. *If the Nitrazine and Fern Test are positive, probable membrane rupture has occurred. ![]() Procedure Notes *The Nitrazine test is highly sensitive but not specific. which indicates a positive result for amniotic fluid. If the pH Nitrazine paper color is blue-green to deep blue, the corresponding pH is 6.5 to 7.5. Results If the pH Nitrazine paper color is yellow to olive green, the corresponding pH is 4.5 to 6.0, which indicates a negative result for amniotic fluid. Immediately match the strip color with the closest color on the dispenser color chart. Nitrazine pH paper test strips should be tested when opening a new container and at least.Apply patient sample to the Nitrazine paper. Procedure Tear off a piece of Nitrazine paper of the desired length. Specimen contamination will result in erroneous pH results. False negative results may be produced by prolonged rupture of membranes (longer than 24 hours) or when a small volume of fluid has leaked. Interferences False positive results may occur from specimen contamination due to heavy vaginal discharge, blood, cervical mucus, semen alkaline urine, and soap. A nitrazine test strip is used to detect the presence of amniotic. ►Test sample immediately after collection. amniotic fluid to leak from the uterus if the membranes are ruptured. ►Do Not touch the swab or the pH paper to the mucus plug in the cervix. Specimen Requirements ►Vaginal secretions from the posterior vaginal pool. *Specimen Requirements *Interferences *Procedure *Results *Procedure Notes The risk is largely eliminated by induction of labor. ![]() Premature rupture of the membranes before onset of labor may lead to fetal infection and subsequent mortality. It is used in conjunction with the Fern Test to help detect ruptured membranes. Nitrazine paper is used to detect small quantities of amniotic fluid in vaginal secretions. Audience: L&D only Author: Tracy Lacy, Medical Technologist Point of Care Coordinator Department: Laboratory, ext 2637 Date: March 2006 ![]()
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